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Extract-N-Amp^™植物PCR试剂盒

Name: Extract-N-Amp<SUP>™</SUP>植物PCR试剂盒
Brand: SIGMA

sufficient for 1000 extractions, sufficient for 1000 amplifications

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别名:

Plant direct PCR kit

用途

sufficient for 1000 amplifications
sufficient for 1000 extractions

特点

hotstart

颜色

colorless

运输

wet ice

储存温度

−20°C

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一般描述

Extract-N-Amp^™植物PCR试剂盒用于直接PCR（聚合酶链反应）。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案，无需常规的植物组织冷冻处理。

应用

Extract-N-Amp^™植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。

Extract-N-Amp^™组织PCR试剂盒用于从新鲜蘑菇中提取DNA。

特点和优势

PCR用植物基因组DNA可以在15分钟内提取。
无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
经过特别配制的PCR ReadyMix 和提取的DNA一起使用
热启动抗体确保基因组DNA的高度特异性PCR扩增
适用于高通量植物基因分析
无需长时间的酶消化，节省时间和精力
提取的DNA在4 °C 下至少6个月保持稳定。

组分

Extract-N-Amp^™植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物（2X）（缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体）。

其他说明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Warning

危险声明

H315 - H317 - H319 - H411

预防措施声明

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

分析证书(COA)

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示例

T1503

货号

25G

包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
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索取COA

Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations.

Antoine Lf Gady et al.

Plant methods, 5, 13-13 (2009-10-09)

The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as

An inexpensive and rapid method for extracting papilionoid genomic DNA from herbarium specimens.

M Riahi et al.

Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)

Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with

A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue.

Dionne N Shepherd et al.

Journal of virological methods, 149(1), 97-102 (2008-02-19)

A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and

Altered starch turnover in the maternal plant has major effects on Arabidopsis fruit growth and seed composition.

Vasilios M E Andriotis et al.

Plant physiology, 160(3), 1175-1186 (2012-09-04)

Mature seeds of both the high-starch starch-excess1 (sex1) mutant and the almost starchless phosphoglucomutase1 mutant of Arabidopsis (Arabidopsis thaliana) have 30% to 40% less lipid than seeds of wild-type plants. We show that this is a maternal effect and is

Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor.

José Dijair Antonino de Souza Júnior et al.

PloS one, 8(12), e85364-e85364 (2014-01-07)

The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita

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商品

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Extract-N-Amp™ Plant Tissue PCR Kits

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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Extract-N-Amp™植物PCR试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

属性

用途

特点

颜色

运输

储存温度

相关类别

说明

一般描述

应用

特点和优势

组分

其他说明

法律信息

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象形图

警示用语：

危险声明

预防措施声明

危险分类

储存分类代码

WGK

闪点(°F)

闪点(°C)

法规信息

文件

分析证书(COA)

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Extract-N-Amp^™植物PCR试剂盒