Skip to Content
Merck
CN

Key Documents

View All Documentation

137265

HEPES sodium salt

EMPROVE® EVOLVE

Synonym(s):

HEPES, Sodium Salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
UNSPSC Code:
12161700
MDL number:
MFCD00036463
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

synthetic

Quality Level

400

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

pH

6.8-8.2

useful pH range

6.8-8.2

pKa (25 °C)

7.5

pKa 

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

Application

HEPES Sodium is a biological buffer often referred to as a “Good′s” buffer. HEPES Sodium, used in conjunction with HEPES, is the most commonly used biological buffer formulation since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Sodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES Sodium is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under HEPES sodium salt

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Stay compliant with GMP using the Emprove® Program

Accelerate qualification, maintain compliance, and save time with Emprove® Dossiers.

Download Dossiers

Note: you will be brought to EmproveSuite.com. Your username and password are separate from SigmaAldrich.com and may differ.

Register for free and access Emprove® Dossiers within minutes. Upgrade your subscription plan to unlock advanced and customized content in the Emprove® Suite

Not finding what you are looking for? Visit the Emprove® Program Help page.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Global Trade Item Number

SKUGTIN
137265010004065267204673
137265901004065267204697
137265100004065267204680
137265902504065267204703