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G3272

Sigma-Aldrich

Guanidine hydrochloride

for molecular biology, ≥99%

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Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
50-01-1
Molecular Weight:
95.53
Beilstein:
3591990
EC Number:
200-002-3
MDL number:
MFCD00013026
PubChem Substance ID:
24895119
NACRES:
NA.31

biological source

synthetic (organic)

grade

for molecular biology

Assay

≥99%

form

crystalline powder

technique(s)

RNA extraction: suitable

impurities

≤0.3% water (Karl Fischer)

color

white

pH

(25 °C, 4.6 - 6/573 g/L)

mp

180-185 °C (lit.)

solubility

H2O: 6 M, clear, colorless

density

1.3 g/cm3 (lit.)

cation traces

Pb: ≤5 ppm

UV absorption

λ: 260 nm Amax: ≤0.10
λ: 290 nm Amax: ≤0.05

foreign activity

DNase, RNase, none detected

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

Gene Information

human ... KCNA1(3736) , KCNA10(3744) , KCNA2(3737) , KCNA3(3738) , KCNA4(3739) , KCNA5(3741) , KCNA6(3742) , KCNA7(3743) , KCNB1(3745) , KCNB2(9312) , KCNC1(3746) , KCNC2(3747) , KCNC3(3748) , KCNC4(3749) , KCND1(3750) , KCND2(3751) , KCND3(3752) , KCNF1(3754) , KCNG1(3755) , KCNG2(26251) , KCNG3(170850) , KCNG4(93107) , KCNH1(3756) , KCNH2(3757) , KCNH3(23416) , KCNH4(23415) , KCNH5(27133) , KCNH6(81033) , KCNH7(90134) , KCNH8(131096) , KCNQ1(3784) , KCNQ2(3785) , KCNQ3(3786) , KCNQ4(9132) , KCNQ5(56479) , KCNS1(3787) , KCNS2(3788) , KCNS3(3790) , KCNV1(27012) , KCNV2(169522)

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Application

Guanidine hydrochloride has been used:
  • as a component of the extraction buffer for the extraction of proteoglycans
  • in extraction during protein fractionation of ATDC5 cell lines
  • as a chemical additive to study its effective absorbance spectra in structural analysis
  • to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer
  • as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study
  • in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain. It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 + H332 - H315 - H319

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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