Merck
CN
All Photos(1)

Documents

Safety Information

MSANGERG

Sigma-Aldrich

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Mouse, Glycerol Format

Sign Into View Organizational & Contract Pricing
All Photos(1)

Quality Level

200

packaging

pkg of 50 μL (96-well plate)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Application

Functional Genomics/Screening /Target Validation
Sanger Arrayed Whole Genome Lentiviral CRISPR Library has been used for Functional Genomics/Screening /Target Validation.

Features and Benefits

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP
  • Flip the expression components into and out of the genome using transposase

Additional Features
  • Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Packaging

96-well plates

Components

Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones

Request a Quote or More Information

Physical form

50 μl of bacterial glycerol stocks, 450×96-well plates

Other Notes

This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Recommended products

Additional libraries are available:
Human, Glycerol Format
Human, Virus Format
Mouse, Virus Format

Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
Emmanouil Metzakopian
Scientific Reports (2017)

Protocols

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service