individual plate number
• Easy documentation and archiving of every plate
• Same reliable performance as TLC and HPTLC plates
manufactured
TLC and HPTLC Plates with Concentrating Zone
Thin-Layer
samples diffuse into the nutrient media and cause growth-free
inhibition zones to develop on the otherwise thickly covered plates. Repeated tests with pH 6,0, pH 8,0
and pH 7,2 are necessary, as penicillin
Interpretation
No zone Clear zone
(17 - 20 mm)
No zone No significant amounts of inhibiting
substances.
Clear zone
(zone
(17 - 20 mm)
No zone significant amounts
block
PickPen® transfers of concentrated samples to prepared resuspension plate
Transfer of resuspended sample to amplification tubes
Work Zone 3 – Amplification & Detection
1.3 and 2.4 Zones.
Sequential cleaning studies ‐ Sequential cleaning
studies were conducted in the laboratory to further
validate the Zones of Cleanliness. Sterile stainless steel
plates were contaminated
sulfamethoxa-
zole and Enterococcus faecalis ATCC 29212.
Calcium and/or magnesium concentrations are controlled by
obtaining the correct zone diameters with aminoglycoside anti-
biotics and Pseudomonas aeruginosa
without a
pink-red colored zone in the medium.
Lactose- and/or sucrose-positive microorganisms produce yellow-green colonies mostly surrounded by
a yellow-green colored zone in the medium.
Colonies of
component
of this sample is saccharose.
Figure 25
TLC-MS Interface (CAMAG, Switzerland)
for the direct extraction of TLC / HPTLC plates.
Figure 26
Compact mass spectrometer
Colonies Microorganisms
Pink surrounded by a red zone Lactose- and sucrose-negative:
Salmonella and others
Yellow-green surrounded by a yellow-green zone Lactose- or sucrose-positive:
E. coli, Citrobacter
and/or VT2) and control (C) zones. A
sample can be considered NEGATIVE if no red line appears in the
test (VT1and VT2) zones but does appear distinctly in the control
(C) zone within 10 - 20 min after
gold-labelled antibody and migrates through the
port until it encounters a binding zone in the test (T) area.
4. The binding zone (T) contains another anti-Salmonella antibody, which immobilises any Salmonella
subtilis on agar plates,
b) using a reference preparation of hygromycin to
establish a standard curve relating to its "zone of
killing" (the area of the bacterial lawn killed)
c) testing a sample
diacylglycerol pyrophosphate
PKA No data
Molec. Formula C39H80N2O11P2 TLC mobile phase C:Acetone:M:Acetic Acid:W*,
50:15:13:12:4, v/v; TLC plate sprayed
with 1% Potassium Oxalate
MW 815.020 Exact Mass 814.524
enhanced the fermentation reaction by increasing the mannitol concentration to 1%
(w/v), clearly indicated by the development of yellow zones surrounding the colonies. S. aureus is able
to reduce tellurite
subtilis on agar
plates,
b) using a reference preparation of hygromycin
to establish a standard curve relating to its
"zone of killing" (the area of the bacterial
lawn killed)
c) testing a sample
15 min. Cool to 50 °C and aseptically add 50 ml concentrated Egg Yolk
Tellurite Emulsion (Cat. No. 75208). Mix well and pour into sterile petri plates. Inoculate the specimen
and streak and incubate
surface of the medium. The plates are incubated
and the zones of inhibition around each disc are measured. The inhibition zones correlate with the
minimal inhibitory concentration (MIC). Different factors
Colonies of Proteus, Morganella, Rettgerella and Providencia are, however, surrounded by dark
brown zones, which occur, because these species act on the phenylalanine of the peptone to
produce phenylpyruvate
gently. Invert the plates and incubate at
35–37 °C in 5–10 % CO2 (microaerophilic) for
18–24 hours using Anaerocult™ C (Cat. No. 132383),
until colony growth is observed. Check for the zone of
inhibition
Accessories
Consistency is our standard
SigmaAldrich.com/TLC
Did you know ...
…that we offer MS-grade TLC and HPTLC plates for rapid identification
and superior characterization using mass spectrometry
can
be stored at 4°C for several weeks. The developed TLC plate is dried, sprayed with anisaldehyde reagent and
heated up slowly on a hot-plate or applying a flameless heat gun until the coloured spots
be stored at 4°C for several weeks. The developed TLC plate is dried, sprayed with anisaldehyde reagent and
heated up slowly on a hot-plate or applying a flameless heat gun until the
64293 Darmstadt
Germany
sigmaaldrich.com
The vibrant M, Merck, Millipore, Assurance and Zones of Cleanliness are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates.
All other trademarks
Supplement (P9602) to a final concentration of 100 units per ml. Add 100 ml of sterile Egg Yolk Emulsion
(Fluka 17148) per 1000 ml of medium. Mix well and pour into sterile petri plates.
Principle and Interpretation
2012/MA
MOUSE ANTI-HUMAN GOLGI ZONE
MONOCLONAL ANTIBODY
CATALOG NUMBER: MAB1271
LOT NUMBER:
QUANTITY: 100 µL
SPECIFICITY: Stains golgi zone area in human cells.
ISOTYPE: IgG1
expressed in key embryonic tissues such
as Hensen’s node, the zone of polarizing activity in the
posterior limb bud, the notochord, and the floor plate of
the neural tube. Downstream targets of Shh include
micro-
graph shows that analyte is concentrated
in a very narrow zone on the membrane
surface.
Analyte
Contaminants
Patented Plate Design
Multi-SPE plates exhibit excellent well-to-well
to an
equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3
nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/10 μM GDP in a
nonbinding 96-well plate. Unlabeled ligand
yellow colonies with yellow zones around the colonies. Typical non-pathogenic staphylococci do
not ferment mannitol and form red colonies. Sodium Chloride, in high concentration, inhibits most
bacteria