Chromatographic conditions of synthesis
control of methyl benzoate.
Conditions
Plate: TLC silica gel 60 F254 MS-grade 5x7.5 cm
Plate:
1. Start of the reaction
2. Reaction after 1 hour
3. Reaction after
downtime.
Use of automated liquid handling
robots with SPE plates offers the ability
to reduce the time and labor involved
in LC-MS/MS sample prep.
High Extraction Efficiency
Eliminates Steps for
Solvents:
The standard precipitation solvent recommended for use with the Hy-
bridSPE-PLus plate is LC-MS grade acetonitrile with 1% formic acid. If
recoveries are less than desired for a specific analyte
T6664 1 packet
EZ-Pierce plate seal Z721581 4 films
Reagents Required but Not Provided.
• 88–91% Formic acid (Catalog Number 399388)
• LC-MS grade water (Catalog Number 1.15333)
T6664 1 packet
EZ-Pierce plate seal Z721581 4 films
Reagents Required but Not Provided.
• 88–91% Formic acid (Catalog Number 399388)
• LC-MS grade water (Catalog Number 1.15333)
shown here. Cerilliant certified reference material grade
standards enabled confident identification. We recommend using
HybridSPE®-Phospholipid plates to extract these compounds from
serum or plasma.
T6664 1 packet
EZ-Pierce plate seal Z721581 4 films
Reagents Required but Not Provided.
88–91% Formic acid (Catalog Number 399388)
LC-MS grade water (Catalog Number 1.15333)
moisture) SU861043
High Flow Special Moisture Filter Kit for 2 filters/base plate SU861045
LC-MS Kit for 2 filter/base plate (2x charcoal:
Nitrogen purification) High Flow without indicator SU861046
Figure 3. MALDI-MS spectra plate load and recovery. Spectrum (A) shows MALDI-MS analysis of trypsin-digested biotinylated lysozyme prior to loading on
Streptavidin HC coated plates. Spectra (B) and (
multiwell
plate. Alternative vials/plates should be validated
prior to use, as non-specific adsorptive losses can
be significant.
LC-MS/MS Analysis
1. Inject 10 L for LC-MS/MS analysis
multiwell
plate. Alternative vials/plates should be validated
prior to use, as non-specific adsorptive losses can
be significant.
LC-MS/MS Analysis
1. Inject 10 L for LC-MS/MS analysis
multiwell
plate. Alternative vials/plates should be validated
prior to use, as non-specific adsorptive losses can
be significant.
LC-MS/MS Analysis
1. Inject 10 L for LC-MS/MS analysis.
the MS-MS detection parameters
Column: Ascentis
® Express Phenyl-Hexyl column
10 cm x 2.1 mm, 2.7 µm (53336-U)
Mobile phase A: LC-MS grade water with 0.1% formic acid
Mobile phase B: LC-MS grade
Chromatography
TLC, HPTLC, TLC-MS, and Accessories
Consistency is our standard
SigmaAldrich.com/TLC
Did you know ...
…that we offer MS-grade TLC and HPTLC plates for rapid identification
and superior
system or LiChrosolv® UHPLC-MS
grade bottled water
ZIQ7000T0
or 1.03728
Acetonitrile for UHPLC-MS LiChrosolv® 1.03725
Trifluoroacetic acid eluent additive for LC-MS,
LiChropur®
80457
Tris
6. In mass spectrometry (MS) applications, the protein
may be digested with proteomics grade trypsin
(Prod. No. T 6567) before affinity purification and
analysis by MS.
7. Capture the biotinylated
66
6. Reference Materials in LC-MS/MS
Applications: Quality Grades &
Selection Considerations
Choosing the correct reference
material quality grade for
your needs
Who uses reference
0.999
mass axis offset 0
plate vias (V) -10
pole vias (V) -10
discriminator (mV) 13
EM voltage (V) -1700
EM final stage (V) -278
Table 2: HP 4500 ICP-MS conditions
post plating, gently shake plates and
remove Fully Supplemented Human Hepatocyte
Plating Medium (HHPM) by gentle vacuum
aspiration and replace with Fully Supplemented
HHCM.
a. Remove plates from the
containing assay protocol, MS Excel file for calculations and data
representation, and material safety data sheets.
4. Equipment and Materials needed
• Plate incubator/shaker.
• Automatic
containing assay protocol, MS Excel file for calculations and data
representation, and material safety data sheets.
4. Equipment and Materials needed
• Plate incubator/shaker.
• Automatic
performance levels for core-type particles with
lowest plate height value. A reduced plate height, h, has no units
and is determined by dividing plate height, H, by average particle
diameter; h values allow
min, and backpressure
was monitored continuously using the system.
Mass spectrometry
LC-MS grade acetonitrile was filtered through the respective syringe filters. The first
and second 1 ml filtrate