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Showing 1-30 of 184 results for "X114" within Papers
Marta Hryniewicka et al.
Journal of pharmaceutical and biomedical analysis, 106, 129-135 (2014-12-10)
This work illustrates the development of new procedures for the isolation and preconcentration of fluvastatin (FLU) from aqueous solutions. Micellar extraction (ME) combined with high performance liquid chromatography (HPLC-UV) has been successfully applied for this purpose. It was found that
Shengchun Yang et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 148, 72-77 (2015-04-13)
In this work, ultrasound-assisted cloud point extraction (UA-CPE) and ultrasound-assisted dispersive liquid liquid microextraction (UA-DLLME) were investigated and compared firstly as ultrasound-assisted liquid phase microextraction methods, which were coupled with spectrophotometer for copper preconcentration and detection. Compared to conventional CPE
Ferial Aslani et al.
Human reproduction (Oxford, England), 30(2), 417-431 (2014-12-03)
Does high mobility group box protein 1 (HMGB1) regulate inflammatory reactions in a rat model of experimental autoimmune orchitis (EAO)? HMGB1 appears to be involved in regulating inflammatory reactions in testes, as HMGB1 is translocated from testicular cells during the
Isolation of integral membrane proteins by phase partitioning with Triton X-114.
J S Brusca et al.
Methods in enzymology, 228, 182-193 (1994-01-01)
Joanna Giebułtowicz et al.
Journal of chromatography. A, 1423, 39-46 (2015-11-18)
Cloud-point extraction (CPE) draws increasing interest in a number of analytical fields including bioanalysis, but combining CPE and LC-MS with electrospray ionization (ESI) in the determination of drugs in biological fluids such as plasma, serum or blood has not been
Paul R Pentel et al.
BMC biotechnology, 18(1), 46-46 (2018-07-26)
The bacterial nicotine-degrading enzyme NicA2 isolated from P. putida was studied to assess its potential use in the treatment of tobacco dependence. Rats were pretreated with varying i.v. doses of NicA2, followed by i.v. administration of nicotine at 0.03 mg/kg. NicA2
R Lappe et al.
Natural product research, 26(23), 2259-2265 (2012-01-21)
Cerein 8A is an antimicrobial peptide with potential application against food spoilage and pathogenic bacteria. The partitioning of cerein 8A was investigated in two liquid-liquid extraction systems that are considered promising for bioseparation and purification purposes. Aqueous two-phase systems (ATPSs)
Brianna Norris-Mullins et al.
Parasitology research, 117(9), 2971-2985 (2018-07-10)
Leishmania parasites are responsible for important neglected diseases in humans and animals, ranging from self-healing cutaneous lesions to fatal visceral manifestations. During the infectious cycle, Leishmania differentiates from the extracellular flagellated promastigote to the intracellular pathogenic amastigote. Parasite differentiation is
Csaba Matta et al.
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 20(8), 572-589 (2016-02-13)
There is insufficient knowledge about the chondrocyte membranome and its molecular composition. To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins. Articular chondrocytes from equine metacarpophalangeal joints were separated into
Mustafa Khan et al.
Ultrasonics sonochemistry, 39, 313-320 (2017-07-25)
A novel and greener ultrasonically assisted/modified cloud point extraction procedure for the simultaneous preconcentration of lead and cadmium in serum samples of different types of gallstone patients was developed. The chelates of the under study metals, formerly formed with 8-hydroxyquinoline
Dimosthenis L Giokas et al.
Journal of chromatography. A, 1251, 33-39 (2012-07-13)
A novel two-step extraction technique combining cloud point extraction (CPE) with dispersive micro-solid phase extraction (D-μ-SPE) is presented in this work for the first time. The method involves initial extraction of the target analytes by CPE in the micelles of
Magnus Monné et al.
Journal of bioenergetics and biomembranes, 49(5), 369-380 (2017-07-12)
The ATP-Mg/phosphate carriers (APCs) modulate the intramitochondrial adenine nucleotide pool size. In this study the concentration-dependent effects of Mg
Qi Zhao et al.
Journal of chromatographic science, 51(4), 341-348 (2012-09-15)
A method based on cloud point extraction (CPE) coupled with high-performance liquid chromatography separation and ultraviolet (UV) detection was developed to determine andrographolide and dehydroandrographolide in human plasma. The nonionic surfactant Triton X-114 was chosen as the extraction medium. Variable
Fabio Andres et al.
Journal of molecular biology, 431(10), 2020-2039 (2019-04-02)
MET, the product of the c-MET proto-oncogene, and its ligand hepatocyte growth factor/scatter factor (HGF/SF) control survival, proliferation and migration during development and tissue regeneration. HGF/SF-MET signaling is equally crucial for growth and metastasis of a variety of human tumors
Aravind Shekar et al.
Medical microbiology and immunology, 208(1), 89-100 (2018-09-14)
Escherichia coli O157:H7 with its traits such as intestinal colonization and fecal-oral route of transmission demands mucosal vaccine development. E. coli secreted protein B (EspB) is one of the key type III secretory system (TTSS) targets for mucosal candidate vaccine
Adam J Lowe et al.
Methods in molecular biology (Clifton, N.J.), 899, 265-275 (2012-06-28)
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method
Maite Lobo et al.
PLoS neglected tropical diseases, 13(3), e0007245-e0007245 (2019-03-15)
TolT was originally described as a Trypanosoma cruzi molecule that accumulated on the trypomastigote flagellum bearing similarity to bacterial TolA colicins receptors. Preliminary biochemical studies indicated that TolT resolved in SDS-PAGE as ~3-5 different bands with sizes between 34 and
Amr Ali Mohamed et al.
Journal of colloid and interface science, 381(1), 198-201 (2012-06-19)
Uniform fully dispersed particles of flufenamic acid, a widely used anti-inflammatory drug, were prepared by two different methods. In the first one, the drug solution in organic solvents was added to a non-solvent (water or aqueous solutions of stabilizers); while
Hannes Steinkellner et al.
Scientific reports, 9(1), 7929-7929 (2019-05-30)
Methyl-CpG-binding protein 2 (MeCP2) is a multifunctional chromosomal protein that plays a key role in the central nervous system. Its levels need to be tightly regulated, as both deficiency and excess of the protein can lead to severe neuronal dysfunction.
Emmanuelle Goubert et al.
Frontiers in cellular neuroscience, 11, 149-149 (2017-06-18)
The solute carrier family 25 (SLC25) drives the import of a large diversity of metabolites into mitochondria, a key cellular structure involved in many metabolic functions. Mutations of the mitochondrial glutamate carrier
C Bordier
The Journal of biological chemistry, 256(4), 1604-1607 (1981-02-25)
A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C. The extent of this detergent phase separation increases with the temperature and
Shikha Gupta et al.
Journal of pharmaceutical and biomedical analysis, 66, 33-39 (2012-04-03)
A new validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitation of four antipsychotic indole alkaloids (IAs), reserpiline (RP, 1), α-yohimbine (YH, 2), isoreserpiline (IRP, 3) and 10-methoxy tetrahydroalstonine (MTHA, 4) as markers in the leaves
Katri Kurppa et al.
Plant biotechnology journal, 16(2), 404-414 (2017-06-24)
Purification is a bottleneck and a major cost factor in the production of antibodies. We set out to engineer a bifunctional fusion protein from two building blocks, Protein A and a hydrophobin, aiming at low-cost and scalable antibody capturing in
Yu-Lin Su et al.
Journal of immunology (Baltimore, Md. : 1950), 194(8), 3997-4007 (2015-03-15)
Helicobacter pylori GroES (HpGroES), a potent immunogen, is a secreted virulence factor that stimulates production of proinflammatory cytokines and may contribute to gastric carcinogenesis. HpGroES is larger than other bacterial orthologs because of an additional C-terminal region, known as domain
Reyhaneh Rahnama et al.
Environmental monitoring and assessment, 188(3), 150-150 (2016-02-10)
A novel improved preconcentration method known as rapidly synergistic cloud point extraction (RS-CPE) was established for nickel preconcentration and determination prior to its determination by flame atomic absorption spectrometry. In this work, the traditional CPE pattern was changed and greatly
Tao Pan et al.
Applied microbiology and biotechnology, 100(1), 431-437 (2015-09-24)
The biological treatment of polycyclic aromatic hydrocarbons is an important issue. Most microbes have limited practical applications because of the poor bioavailability of polycyclic aromatic hydrocarbons. In this study, the extractive biodegradation of phenanthrene by Sphingomonas polyaromaticivorans was conducted by
Nam Joong Kim et al.
Molecular immunology, 65(1), 17-24 (2015-01-21)
Staphylococcus aureus is a Gram-positive bacterium that causes inflammation at infection sites by inducing various inflammatory mediators such as nitric oxide (NO). To identify the staphylococcal virulence factors contributing to NO production, we compared the ability of ethanol-killed wild-type S.
Flemming Jessen et al.
Analytical biochemistry, 485, 11-17 (2015-06-17)
A simple and reproducible procedure for enrichment of a plasma protein subfraction suitable for two-dimensional polyacrylamide gel electrophoresis (2DE) was developed, using a Triton X-114-based cloud point extraction (CPE). Appropriate conditions for such a CPE procedure were found by SDS-PAGE
Ester Peris-García et al.
Analytical and bioanalytical chemistry, 410(20), 5043-5057 (2018-06-21)
Most reports in reversed-phase liquid chromatography (RPLC) with micellar mobile phases make use of the anionic sodium dodecyl sulfate. This surfactant masks efficiently the silanol groups that are the origin of the poor efficiencies and tailing peaks observed for basic
Hamta Shoaee et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 98, 70-75 (2012-09-18)
A cloud-point extraction process coupled to ICP-OES by using 3-nitro benzaldehyde thiosemicarbazone (3-NBT) as complexing agent was developed for the simultaneous preconcentration and determination of copper and mercury in water samples. The variables affecting the complexation and extraction steps were
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