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安全信息

WGA1

Sigma-Aldrich

GenomePlex® Whole Genome Amplification (WGA) Kit

Kit for whole genome amplification from a variety of DNA sources including FFPE tissue

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NACRES:
NA.55

技术

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

相关类别

一般描述

GenomePlex® 全基因组扩增 WGA 试剂盒适用于基因组DNA、全血、白细胞层（buffy coat）、口腔拭子和培养的细胞。 扩增只需要少量起始原料（1 μL 血、单面颊拭子或10 ng gDNA），PCR后每次反应可得到5 - 10 μg的产量 。 扩增的DNA 可进行纯化和储存或用于下游分析，包括ABI′s TaqMan®测定、微卫星分析或测序。 GenomePlex 完整表达整个基因组，等位基因偏差最小。

WGA试剂盒采用基于基因组 DNA 随机片段化的专有技术，并将产生的小片段转换为两侧有通用引物结合位点的 PCR 可扩增文库分子。 WGA是利用通用寡核苷酸引物对文库分子进行PCR扩增获得的。

GenomePlex 方法让研究人员可以生成具有代表性的、约 500 倍扩增的基因组 DNA 。 扩增产量取决于纯度和起始原料的量。该试剂盒含有进行文库制备和片段化需要的所有试剂。

应用

GenomePlex ® 全基因组扩增 (WGA) 试剂盒已用于扩增输入和染色质免疫沉淀 (ChIP) DNA。它也用于来源于细菌培养物的细菌人工染色体（BAC）克隆体的片段化，用于制备无重复（repeat free，RF）探针。

特点和优势

  • 以最少的模板获得更高的产量: 在三小时以内将纳克量（10ng）的基因组DNA扩增为微克产量（5-10 μg）
  • 纳克量样品可在–20 °C下保存备用
  • 从各种DNA来源中选择：全血、口腔拭子、 血卡、植物、土壤、福尔马林固定石蜡包埋组织（FFPE）
  • 兼容各种下游应用，如TaqMan®和BeadArray 测定
  • 通用引物
  • 表达全基因组，没有可检测的偏差
  • 增加的扩增准确性不会在阴性对照反应中产生扩增子

其他说明

该试剂盒随附的通用引物的序列组成具有专利权。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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  1. What is the major difference between WGA1 and WGA2?

    Functionally WGA1 and WGA2 are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.

  2. If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with WGA1, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?

    The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.  

  3. Can I use WGA1, GenomePlex® Amplification Kit to amplify DNA from a single cell?

    Using this kit to amplify DNA from a single cell is not recommended.  We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application.  WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.

  4. Can I amplify single stranded DNA with Product WGA, GenomePlex® Amplification Kit?

    When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20.  Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.  

  5. Is the WGA1 GenomePlex®  Amplicification Kit polymerase compatible with TA cloning?

    WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.

  6. What is the major difference between WGA1 and WGA2 kits?

    Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.

  7. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Adam Hittelman et al.
Diagnostic molecular pathology : the American journal of surgical pathology, part B, 16(4), 198-206 (2007-11-29)
Genome-based technologies such as genomic arrays and next generation sequencing are poised to make significant contributions to clinical oncology. However, translation of these technologies to the clinic will require that they produce high-quality reproducible data from small archived tumor specimens
Amy Breman et al.
Prenatal diagnosis, 32(4), 351-361 (2012-04-03)
To evaluate the results of prenatal chromosomal microarray analysis (CMA) on >1000 fetal samples referred for testing at our institution and to compare these data to published reports. High resolution CMA was offered to women undergoing amniocentesis or chorionic villus
Kaye N Ballantyne et al.
Forensic science international, 166(1), 35-41 (2006-05-12)
The analysis of LCN or highly degraded DNA samples presents a challenge for forensic science. Improving the quantity and/or quality of samples would greatly increase the profiling success rate from LCN and degraded samples. Whole genome amplification (WGA) is one
Whole genome amplification and genetic analysis after extraction of proteins from dried blood spots.
Mads V Hollegaard et al.
Clinical chemistry, 53(6), 1161-1162 (2007-05-23)
Li Jia et al.
PLoS genetics, 5(8), e1000597-e1000597 (2009-08-15)
Multiple discrete regions at 8q24 were recently shown to contain alleles that predispose to many cancers including prostate, breast, and colon. These regions are far from any annotated gene and their biological activities have been unknown. Here we profiled a
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实验方案

Whole Gene Amplification Formalinfixed Paraffin embedded Tissues

Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.

Whole Gene Amplification Formalinfixed Paraffin embedded Tissues

Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.

FFPE Tissue

Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.

FFPE 组织

存档的福尔马林固定和石蜡包埋 (FFPE) 组织样本是分析基因表达和研究多种疾病的宝贵资源。

查看所有结果

相关内容

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

动物组织DNA提取和WGA扩增实验方案

GenomePlex®全基因组扩增是从动物样品中提取DNA的方法。GenomePlex®产品已用于扩增鸡、猪、牛、鱼和虾来源的基因组DNA。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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