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Preparation of Competent Cells and Transformation with pGEX DNA
This page describes preparation of competent cells and transformation with pGEX DNA.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Study: Comparison of MC-Media Pad® with Different Convenience Solution Products
Comparison of MC-Media Pad® with different convenience solution products for indicator organism testing
ARGET ATRP: Procedure for PMMA Polymer Brush Growth
We presents an article about ARGET ATRP, and its procedure for PMMA polymer brush growth. Surface preparation before polymer brush growth consists of two steps: surface cleaning and initiator monolayer deposition.
寡核苷酸退火实验方案
退火是加热和冷却两条具有互补序列的单链寡核苷酸的过程。热量会破坏所有氢键，而冷却则会在序列之间形成新的键。
用于核酸去磷酸化的小牛肠（CIP）碱性磷酸酶
CIP可用于脱去DNA、RNA及核糖和脱氧核糖核苷三磷酸上的5’-磷酸基团。如何进行DNA去磷酸化的详细实验方案。
用于亲和层析的色谱柱填充和制备实验方案
该页面展示了如何使用GE医疗所提供的Tricorn™或XK色谱柱进行用于亲和层析的色谱柱填充和制备。
Typical Procedures for Polymerizing via RAFT
We presents an article featuring procedures that describe polymerization of methyl methacrylate and vinyl acetate homopolymers and a block copolymer as performed by researchers at CSIRO.
Single-Walled Carbon Nanotubes: Exfoliation and Debundling Procedure
The dispersibility and bundle defoliation of single-walled carbon nanotubes (SWCNTs), which can be applied to materials produced by the CoMoCAT® process, have been extensively investigated by SouthWest NanoTechnologies (SWeNT ®) and at the University of Oklahoma.
人冠状动脉内皮细胞(HCAEC)培养方案
将细胞放入冻存管后须立即储存在液氮储罐中。
Extracellular Vesicle Protocol
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Bestmann-Ohira-试剂：从醛合成炔烃的实验方案
乙炔化学一直以来都是分子科学的重要组成部分。其在有机合成、材料科学、生物有机化学一系列领域都具有极大的潜力，可用在多种应用中。部分实例包括烯二炔（DNA-裂解剂）、“点击化学”工具和嵌块。因此，这些特性引发了对高效合成炔烃的需求。
Affinity Purification with Ultrafree®-MC Centrifugal Filters
Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step
Lipid Transfection Optimization Protocol
Expert guidance on the best way to optimize your transfection experiments. Optimized protocols result in highest efficiency transfections.
Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Traceless Staudinger Ligation
Traceless Staudinger Ligation
Duolink® PLA Probemaker指南
用于免疫荧光或明场检测的定制PLA探针的创建指南。
Production of 15N-13C Dual-labelled Proteins
Dr. Alan Robertson and Dr. Ben Davis at Vernalis, Ltd., UK, a provider of drug discovery and development services, have refined a novel protocol for the duallabelling of proteins using EnPresso® B Defined Nitrogen-free.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti® Polar Lipids
This procedure describes the preparation of Cationic Lipid/Oligomer Complex.
Human Fibroblast-Like Synoviocytes (HFLS) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Prestige Antigens™—封闭实验方案
推荐按照标准的Prestige Antigen封闭实验方案使用Prestige Antigens™以及相应的Prestige Polyclonals或Monoclonals
Multiplex qPCR Protocol
A protocol that can be used as a basic template for qPCR incorporating up to four detection probes. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt ReadyMix.
使用SYBR Green I染料检测的qPCR基因表达/拷贝数分析实验方案
使用 SYBR Green I 染料检测一个或多个稳定内参基因的靶量是qPCR的常见应用。下面是一个可以适应特定实验需要的标准实验方案。
微生物生长实验方案
在微生物培养基中生长感受态细胞的常规实验方案。
Neural Stem Cell Culture Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Better Tools for Fast and Convenient Patulin Analysis
For a precise detection of patulin, we offer SPE cartridges for purification and a comprehensive range of analytical standards.
Genomic DNA Purification using illustra™ GenomicPrep Mini Spin Kits
This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from GE Healthcare.
Canine Aortic Endothelial Cells (CnAOEC) Culture Protocol
Technical information for working with Canine Aortic Endothelial Cells including thawing, subculturing and cryopreservation
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