PCR

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facet applications:PCR

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Learn about the benefits of using Hot Start PCR reagents to suppress enzymatic activity before PCR reaction tubes enter the thermal cycler.

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis

Mycoplasma PCR ELISA Protocol

Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma

Long and Accurate PCR

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

GC-RICH PCR System Troubleshooting

GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

Preserved samples from medical, forensic, museum and other archival collections represent a rich source of study material, much of it meticulously collected, characterized and preserved through many decades of work by experts in the field.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®

Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that

锁核酸

锁核酸 (LNA) 常见问题解答

Using PCR to Detect Viral Agents in Animal Sera

The PCR method for detection of viral agents in FBS and other animal sera can help to ensure virus-free serum for cell culture.

ThermaGenix产品概述

如何在PCR中摆脱非特异性条带和脱靶引物。

Dual-Labeled Probes in Digital PCR

Digital PCR (dPCR) is an end-point PCR method that is used for absolute quantification and for analysis of minority sequences against a background of similar majority sequences.

定量聚合酶链反应和数字聚合酶链反应的检测方法

荧光染料或探针包含在PCR混合物中，用以监测在反应进行过程中DNA扩增子浓度的变化。

KAPA Express Extract Kit FAQs

Frequently asked questions (FAQs) for KAPA Express Extract Kit.

使用PCR

一般考虑因素；RT-PCR中要考虑的因素；防止残留污染；故障排除

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

Oligonucleotide Quality Control by Mass Spectrometry

Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.

ThermaStop^™-RT PCR Additive

PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.

寡核苷酸质量控制和质量保证

Sigma制造流程的基础在于一套稳健的质量管理体系，其推动了向ISO 9001:2008认证的合规性。

维甲酸与基因表达

全反式维甲酸(RA, ATRA)是一种多效激活因子，负责调节与正常脊椎动物细胞分化、细胞增殖、凋亡和胚胎发育等过程相关的基因。

KAPA2G Robust PCR Kits FAQs

Frequently asked questions (FAQs) for KAPA2G Robust PCR Kits.

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA

Precision Genetic Modifications

Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address

Polymerase Chain Reaction (PCR) Basics

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

DNA寡核苷酸合成

了解我们用于DNA寡核苷酸生产的寡核苷酸固相亚磷酰胺合成法的步骤。

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

Roche PCR Reagents and PCR Protocols

Roche polymerase chain reaction (PCR) reagents for reducing the error rate of Taq DNA polymerase and PCR selection guide.

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