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L4391

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

γ-irradiated, BioXtra, suitable for cell culture

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Synonym(s):
LPS
EC Number:
297-473-0
MDL number:
MFCD00164401
NACRES:
NA.75

Quality Level

300

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

storage condition

desiccated

technique(s)

cell culture | mammalian: suitable

impurities

<1% Protein (Lowry)

solubility

H2O: 5 mg/mL, slightly hazy

storage temp.

2-8°C

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Application

This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

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Description
Pricing

L4391

Lipopolysaccharides from Escherichia coli O111:B4, γ-irradiated, BioXtra, suitable for cell culture

L3012

Lipopolysaccharides from Escherichia coli O111:B4, purified by gel-filtration chromatography

L4130

Lipopolysaccharides from Escherichia coli O111:B4, purified by trichloroacetic acid extraction

L3024

Lipopolysaccharides from Escherichia coli O111:B4, purified by ion-exchange chromatography, TLR ligand tested

L2630

Lipopolysaccharides from Escherichia coli O111:B4, purified by phenol extraction

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Lipopolysaccharides from Escherichia coli O111:B4, Detoxified

F3665

Lipopolysaccharides from Escherichia coli O111:B4, FITC conjugate

L5293

Lipopolysaccharides from Escherichia coli O111:B4, Ready Made solution, 1 mg/mL

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Jennifer Vandooren et al.
Haematologica, 102(10), 1671-1682 (2017-08-05)
Lipopolysaccharides or endotoxins elicit an excessive host inflammatory response and lead to life-threatening conditions such as endotoxemia and septic shock. Lipopolysaccharides trigger mobilization and stimulation of leukocytes and exaggerated production of pro-inflammatory molecules including cytokines and proteolytic enzymes. Matrix metalloproteinase-9
Kelly L Brown et al.
Journal of immunology (Baltimore, Md. : 1950), 186(9), 5497-5505 (2011-03-29)
The human cathelicidin peptide, LL-37, is a host defense peptide with a wide range of immunomodulatory activities and modest direct antimicrobial properties. LL-37 can exert both pro- and anti-inflammatory effects and can modulate the proinflammatory responses of human peripheral blood
Chuan Yan et al.
Journal of hepatology, 63(2), 420-428 (2015-04-02)
Chronic inflammation is a major etiological factor for hepatocellular carcinoma (HCC), but how immune cells respond in the initiation of hepatocarcinogenesis remains uncharacterized. This study aims to investigate the response and roles of neutrophils in early hepatocarcinogenesis. By inducible expression
Wei Shao et al.
The Journal of biological chemistry, 282(50), 36321-36329 (2007-10-26)
Caspase-1 is an essential effector of inflammation, pyroptosis, and septic shock. Few caspase-1 substrates have been identified to date, and these substrates do not account for its wide range of actions. To understand the function of caspase-1, we initiated the
Christopher R Shaler et al.
PLoS biology, 15(6), e2001930-e2001930 (2017-06-21)
Superantigens (SAgs) are potent exotoxins secreted by Staphylococcus aureus and Streptococcus pyogenes. They target a large fraction of T cell pools to set in motion a "cytokine storm" with severe and sometimes life-threatening consequences typically encountered in toxic shock syndrome
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