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L6143

Sigma-Aldrich

Lipopolysaccharides from Salmonella enterica serotype typhimurium

suitable for cell culture, BioXtra, γ-irradiated

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Synonym(s):
LPS
MDL number:
MFCD00164404
NACRES:
NA.75

Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

technique(s)

cell culture | mammalian: suitable

impurities

<1% Protein (Lowry)

storage temp.

2-8°C

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Application

Lipopolysaccharides from Salmonella enterica serotype typhimurium has been used:
  • in mice to elicit the secretion of pro-inflammatory cytokines
  • to induce cytokine production in monocytes
  • to elicit the activation of NF-κB pathway in mouse dendritic cells.
  • to induce inflammatory response in human colon adenocarcinoma Caco-2 cells
  • for stimulation of the catfish peripheral blood leukocytes (PBLs) to study estrogen receptor mRNA expression
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 + H312 + H332

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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