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R4251

Sigma-Aldrich

Ribonucleic acid, transfer from Escherichia coli

Type XXI, Strain W, lyophilized powder

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Synonym(s):
Transfer RNA, tRNA
CAS Number:
9014-25-9
MDL number:
MFCD00132200

type

Type XXI

form

lyophilized powder

storage temp.

−20°C

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Application

Ribonucleic acid, transfer from Escherichia coli, Type XXI, Strain W may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

Biochem/physiol Actions

Transfer RNA is an amino acid acceptor in the reaction with Aminoacyl-tRNA Synthetase and various amino acids, resulting in the formation of aminoacyl-tRNA.

Unit Definition

One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).

Regulatory Information

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Jean Lehmann et al.
Nucleic acids research, 41(10), 5494-5502 (2013-04-13)
The structure and function of conserved motifs constituting the apex of Stem I in T-box mRNA leaders are investigated. We point out that this apex shares striking similarities with the L1 stalk (helices 76-78) of the ribosome. A sequence and
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Journal of medicinal chemistry, 57(13), 5566-5578 (2014-06-25)
Lead optimization focuses on binding-affinity improvement. If a flat structure-activity relationship is detected, usually optimization strategies are abolished as unattractive. Nonetheless, as affinity is composed of an enthalpic and entropic contribution, factorization of both can unravel the complexity of a
Tanja Kalstrup et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(20), 8272-8277 (2013-05-01)
Atomic-scale models on the gating mechanism of voltage-gated potassium channels (Kv) are based on linear interpolations between static structures of their initial and final state derived from crystallography and molecular dynamics simulations, and, thus, lack dynamic structural information. The lack
Narayana Annaluru et al.
Science (New York, N.Y.), 344(6179), 55-58 (2014-03-29)
Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair
Stefan Klumpp et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(42), 16754-16759 (2013-10-02)
Bacterial growth is crucially dependent on protein synthesis and thus on the cellular abundance of ribosomes and related proteins. Here, we show that the slow diffusion of the bulky tRNA complexes in the crowded cytoplasm imposes a physical limit on
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