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CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Enzymatic Assay of Cholesterol Oxidase

Solubilizing Peptides

Solubilizing Custom Peptides

cAMP Assays

cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

This procedure may be used for Alcohol Dehydrogenase products.

Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

FFPE Tissue

Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.

Extracellular Vesicle Protocol

Learn best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.

SnAP Reagents

Saturated N-heterocyclic building blocks or SnAP Reagents are of growing importance for the convenient synthesis of medium-ring saturated N-heterocycles, including bicyclic and spirocyclic structures. SnAP reagents are stable and readily available and can be coupled with widely available aromatic, heteroaromatic

DIG Easy Hyb™ Granules Protocol & Troubleshooting

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

Fabrication of Poly(triaryl amine) Field-effect Transistors

Protocols for the Fmoc SPPS of Cysteine-containing Peptides

Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine.

Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit

The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Explant Sterilization – Plant Tissue Culture Protocol

Explant Sterilization, Orchid Seed Sterilization

Prestige Antigens™—Blocking Protocol

Prestige Antigens™ and the corresponding Prestige Polyclonals or Monoclonals are recommended to use according to the standard Prestige Antigen-blocking protocol

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Cell culture protocol for passaging and splitting semi-adherent cell lines using trypsin EDTA. Free ECACC handbook download.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Kit for Creating Hydrophilic PDMS Surfaces

Preparation of Competent Cells and Transformation with pGEX DNA

This page describes preparation of competent cells and transformation with pGEX DNA.

Extract-N-Amp™ FFPE Tissue PCR Protocol

Column Packing and Preparation

This page describes efﬁcient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.

Enzymatic Method for Determining Glucose and Sucrose (Glucose and Sucrose Assay)

Assay protocol for the colorimetric/fluorometric detection of glucose and sucrose in biological samples using the glucose and sucrose assay kit.

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