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PCR
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facet applications:PCR
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SEQR – SeqPlex RNA扩增试剂盒实验方案
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
Hot Start dNTP protocol to reduce non-specific amplification
Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
Titan™ One Tube RT-PCR System Protocol & Troubleshooting
Titan One Tube RT-PCR System Protocol & Troubleshooting
Rapid Genotyping of Mouse Tissue Using Extract-N-Amp Tissue PCR Kit
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending
Amplification of Genomic DNA using REDTaq DNA Polymerase
Method for PCR using genomic DNA or other complex templates
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
寡核苷酸退火实验方案
退火是加热和冷却两条具有互补序列的单链寡核苷酸的过程。热量会破坏所有氢键，而冷却则会在序列之间形成新的键。
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
TeloTAGGG Telomerase PCR ELISA Protocol & Troubleshooting
The TeloTAGGG Telomerase PCR ELISA is used for sensitive qualitative detection of telomerase activity.
Whole Genome Amplification (WGA) Using GenomiPhi™
The GenomiPhi™ method of WGA from GE Healthcare uses Phi29 DNA polymerase, a highly processive enzyme with excellent strand-displacement activity, in combination with random-sequence hexamer primers (random hexamers) to amplify DNA in an isothermal process—a thermal cycler is not required.
Extraction & Amplification of Whole Blood Using WGA-Protocol
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged
HighPrep™ PCR Clean Up Kit and Purification System Protocol
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
dNTP Mediated Hot Start PCR Protocol
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Expand™ Long Template PCR System Protocol & Troubleshooting
To meet the challenges of long range PCR we have developed the second generation kit, the Expand Long Range dNTPack. This kit includes the same enzyme mix, but provides more flexibility: The Expand Long Range dNTPack is provided with two
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase
Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.
Whole Blood
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
REDTaq™应用
介绍RedTaq的应用和优势，包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。
Extract-N-Amp™ Plant PCR Kits Protocol
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)
Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization
Circular DNA Amplification using TempliPhi™
This page shows circular DNA amplification using TempliPhi™ from GE Healthcare.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
完整全转录组扩增试剂盒实验方案(WTA2)
WTA2（一种全转录组扩增（WTA）方法）能够在区区4小时内，代表性地扩增出纳克级的总RNA而没有3'偏差。
REDExtract-N-Amp™组织PCR试剂盒实验方案
exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法，可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。
使用Jumpstart™Taq DNA聚合酶(D9307)扩增DNA
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
Digital PCR
数位PCR是终点PCR方法，其用于绝对定量和针对相似多数序列的背景分析少数序列，例如体细胞突变的定量。
Antibody-Enzyme Mediated Hot Start PCR Protocol
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Extract-N-Amp™ FFPE Tissue PCR Protocol
Protocol for PCR amplification of formalin-fixed paraffin embedded animal tissues using the Extract-N-Amp Tissue PCR kit.
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